Improved control of partial DNA restriction enzyme digest in agarose using limiting concentrations of Mg+ +

Improved control of partial DNA restriction enzyme digest in agarose using limiting concentrations of Mg++.

Partial digests of DNA for cloning experiments are usually performed using either the quantity of restriction enzyme or incubation time, or both, as controlling factors. However, we have found that more precise and reproducible partial digests are obtained, when a limiting concentration of the required cofactor Mg" 1 "" 1 " is used. This method is especially useful for partial digests of DNA co...

Dual restriction enzyme digest of cationic-gold-coated DNA scaffolds

DNA strands coated with AuNPs were cleaved by restriction enzymes while in solution or on a surface. Enzymatic activity was verified by gel electrophoresis prior to surface analysis. Cleavage results suggest that enzymes can recognize the AuNP-coated strands while on the surfaces, though specificity in digestion has not yet been verified. Development allows for advances in site specific localiz...

A Partial Digest Approach to Restriction

We present a new, practical algorithm to resolve the experimental data in restriction site analysis, which is a common technique for mapping DNA. Speciically, we assert that multiple digestions with a single restriction enzyme can provide suucient information to identify the positions of the restriction sites with high probability. The motivation for the new approach comes from combinatorial re...

determination of olanzapine and thiourea using electrodes modified by dna and film of copper-cobalt hexacyanoferrate & investigation of electro-oxidation of some catechol derivatives in the presence of 4-phenylsemicarbazid

چکیده هدف از این کار بررسی الکترواکسیداسیون کتکول و مشتقات آن در حضور 4-فنیل سمی کاربامازید بوده است اکسیداسیون کتکولها ترکیبات نا پایدار کینونها را تولید می کنند که این ترکیبات می تواند در واکنش مایکل بعنوان پذیرنده نوکلئوفیل عمل نمایند. در ادامه اکسایش کتکولهای (a-c1) را درحضور 4-فنیل سمی کاربامازید در محلول آب/استونیتریل (90/10)بوسیله ولتامتری چرخه ای و کولن متری در پتانسیل ثابت مورد بررسی ...

Characterization of Avian Pasteurella multocida Isolates by Protein Profiles and Restriction Enzyme Analysis of Chromosomal DNA